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Droplet microarrays for cell culture: effect of surface properties and nanoliter culture volume on global transcriptomic landscape

Chakraborty, S.; Gourain, V.; Benz, M.; Scheiger, J. M.; Levkin, P. A. ORCID iD icon; Popova, A. A.

Abstract:

The development of novel chemically developed and physically defined surfaces and environments for cell culture and screening is important for various biological applications. The Droplet microarray (DMA) platform based on hydrophilic-superhydrophobic patterning enables high-throughput cellular screening in nanoliter volumes and on various biocompatible surfaces. Here we performed phenotypic and transcriptomic analysis of HeLa-CCL2 cells cultured on DMA, with a goal to analyze cellular response on different surfaces and culture volumes down to 3 nL, compared with conventional cell culture platforms. Our results indicate that cells cultured on four tested substrates: nanostructured nonpolymer, rough and smooth variants of poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) polymer and poly(thioether) dendrimer are compatible with cells grown in Petri dish. Cells cultured on nanostructured nonpolymer coating exhibited the closet transcriptomic resemblance to that of cells grown in Petri dish. Analysis of cells cultured in 100, 9, and 3 nL media droplets on DMA indicated that all but cells grown in 3 nL volumes had unperturbed viability with minimal alterations in the transcriptome compared with 96-well plate. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000133834
Veröffentlicht am 14.06.2021
Originalveröffentlichung
DOI: 10.1016/j.mtbio.2021.100112
Scopus
Zitationen: 3
Dimensions
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2590-0064
KITopen-ID: 1000133834
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Weitere HGF-Programme 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Materials Today Bio
Verlag Elsevier
Band 11
Seiten Art.-Nr.: 100112
Vorab online veröffentlicht am 30.04.2021
Nachgewiesen in Scopus
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