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Phytochrome Mediated Responses in Agrobacterium fabrum: Growth, Motility and Plant Infection

Xue, P. 1; Bai, Y. 1; Rottwinkel, G. 1; Averbukh, E. 1; Ma, Y.; Roeder, T.; Scheerer, P.; Krauß, N. 1; Lamparter, T. 1
1 Karlsruher Institut für Technologie (KIT)

Abstract:

The soil bacterium and plant pathogen Agrobacterium fabrum C58 has two phytochrome photoreceptors, Agp1 and Agp2. We found that plant infection and tumor induction by A. fabrum is down-regulated by light and that phytochrome knockout mutants of A. fabrum have diminished infection rates. The regulation pattern of infection matches with that of bacterial conjugation reported earlier, suggesting similar regulatory mechanisms. In the regulation of conjugation and plant infection, phytochromes are active in darkness. This is a major difference to plant phytochromes, which are typically active after irradiation. We also found that propagation and motility were affected in agp1− and agp2− knockout mutants, although propagation was not always affected by light. The regulatory patterns can partially but not completely be explained by modulated histidine kinase activities of Agp1 and Agp2. In a mass spectrometry-based proteomic study, 24 proteins were different between light and dark grown A. fabrum, whereas 382 proteins differed between wild type and phytochrome knockout mutants, pointing again to light independent roles of Agp1 and Agp2.


Verlagsausgabe §
DOI: 10.5445/IR/1000133883
Veröffentlicht am 14.06.2021
Originalveröffentlichung
DOI: 10.1007/s00284-021-02526-5
Scopus
Zitationen: 2
Web of Science
Zitationen: 2
Dimensions
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0343-8651, 1432-0991
KITopen-ID: 1000133883
Erschienen in Current Microbiology
Verlag Springer
Band 78
Heft 7
Seiten 2708–2719
Nachgewiesen in Dimensions
Scopus
Web of Science
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