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3D Cultivation Techniques for Primary Human Hepatocytes

Bachmann, Anastasia; Moll, Matthias; Gottwald, Eric; Nies, Cordula; Zantl, Roman; Wagner, Helga; Burkhardt, Britta; Sánchez, Juan; Ladurner, Ruth; Thasler, Wolfgang; Damm, Georg; Nussler, Andreas

Abstract:
One of the main challenges in drug development is the prediction of in vivo toxicity based on in vitro data. The standard cultivation system for primary human hepatocytes is based on monolayer cultures, even if it is known that these conditions result in a loss of hepatocyte morphology and of liver-specific functions, such as drug-metabolizing enzymes and transporters. As it has been demonstrated that hepatocytes embedded between two sheets of collagen maintain their function, various hydrogels and scaffolds for the 3D cultivation of hepatocytes have been developed. To further improve or maintain hepatic functions, 3D cultivation has been combined with perfusion. In this manuscript, we discuss the benefits and drawbacks of different 3D microfluidic devices. For most systems that are currently available, the main issues are the requirement of large cell numbers, the low throughput, and expensive equipment, which render these devices unattractive for research and the drug-developing industry. A higher acceptance of these devices could be achieved by their simplification and their compatibility with high-throughput, as both aspects are of major importance for a user-friendly device.

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Verlagsausgabe §
DOI: 10.5445/IR/1000133937
Veröffentlicht am 15.06.2021
Originalveröffentlichung
DOI: 10.3390/microarrays4010064
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 2076-3905
KITopen-ID: 1000133937
Erschienen in Microarrays
Verlag MDPI
Band 4
Heft 1
Seiten 64–83
Vorab online veröffentlicht am 16.02.2015
Schlagwörter primary human hepatocytes; three-dimensional (3D) cell culture; two-dimensional (2D) cell culture; in vitro model; hydrogels; scaffolds; drug-induced hepatotoxicity; long-term culture
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