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Designing Inherently Photodegradable Cell‐Adhesive Hydrogels for 3D Cell Culture

Rosenfeld, Alisa 1; Göckler, Tobias 2; Kuzina, Mariia 1; Reischl, Markus ORCID iD icon 3; Schepers, Ute 2,4; Levkin, Pavel A. ORCID iD icon 4
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
3 Institut für Automation und angewandte Informatik (IAI), Karlsruher Institut für Technologie (KIT)
4 Institut für Organische Chemie (IOC), Karlsruher Institut für Technologie (KIT)


Light-based microfabrication techniques constitute an indispensable approach to fabricate tissue assemblies, benefiting from noncontact spatially and temporarily controlled manipulation of soft matter. Light-triggered degradation of soft materials, such as hydrogels, is important in tissue engineering, bioprinting, and related fields. The photoresponsiveness of hydrogels is generally not intrinsic and requires complex synthetic procedures wherein photoresponsive crosslinking groups are incorporated into the hydrogel. This paper demonstrates a novel biocompatible and inherently photodegradable poly(ethylene glycol) methacrylate (PEGMA)-based gelatin-methacryloyl (GelMA)-containing hydrogel that can be used to culture cells in 3D for at least 14 d. These gels are conveniently and quickly degraded via UV irradiation for 10 min to produce structured hydrogels of various geometries, sizes, and free-standing cell-laden hydrogel particles. These structures can be flexibly produced on demand. In particular, photodegradation can be temporarily delayed from photopolymerization, offering an alternative to hydrogel array production via photopolymerization with a photomask. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000133945
Veröffentlicht am 15.06.2021
DOI: 10.1002/adhm.202100632
Zitationen: 10
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Institut für Biologische und Chemische Systeme (IBCS)
Institut für Funktionelle Grenzflächen (IFG)
Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 18.08.2021
Sprache Englisch
Identifikator ISSN: 2192-2640, 2192-2659
KITopen-ID: 1000133945
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Weitere HGF-Programme 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Advanced healthcare materials
Verlag Wiley-VCH Verlag
Band 10
Heft 16
Seiten Art.Nr. 2100632
Vorab online veröffentlicht am 10.06.2021
Nachgewiesen in Dimensions
Web of Science
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