KIT | KIT-Bibliothek | Impressum | Datenschutz

Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis

Edel, Miriam 1; Sturm, Gunnar 2; Sturm-Richter, Katrin 1; Wagner, Michael 2; Ducassou, Julia Novion; Couté, Yohann; Horn, Harald 3,4; Gescher, Johannes 1,2
1 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)
3 Engler-Bunte-Institut (EBI), Karlsruher Institut für Technologie (KIT)
4 Institut für Technische Chemie und Polymerchemie (ITCP), Karlsruher Institut für Technologie (KIT)

Abstract:

Background
Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. This process can be applied in bioelectrochemical systems in which the organisms produce an electrical current by respiring with an anode as electron acceptor. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalyzed by the terminal reductases and in some cases as endogenous electron shuttles.

Results
In the model organism Shewanella oneidensis, riboflavin production and excretion trigger a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum-sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescine decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. Using a model of growth-dependent riboflavin production under batch and biofilm growth conditions, the number of cells necessary to produce the threshold concentration per time was deduced. Furthermore, our results indicate that specific retention of riboflavin in the biofilm matrix leads to localized concentrations, which by far exceed the necessary threshold value.
... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000133966
Veröffentlicht am 20.09.2021
Originalveröffentlichung
DOI: 10.1186/s13068-021-01981-3
Scopus
Zitationen: 30
Web of Science
Zitationen: 27
Dimensions
Zitationen: 31
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1754-6834
KITopen-ID: 1000133966
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Biotechnology for Biofuels
Verlag Springer Fachmedien Wiesbaden
Band 14
Heft 1
Seiten 130
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Dimensions
Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page