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Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment

Azarfar, Ghazal; Aboualizadeh, Ebrahim; Ratti, Simona; Olivieri, Camilla; Norici, Alessandra; Nasse, Michael J. 1; Giordano, Mario; Hirschmugl, Carol J.
1 Karlsruher Institut für Technologie (KIT)

Abstract:

Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 minutes. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000134622
Veröffentlicht am 01.07.2021
Originalveröffentlichung
DOI: 10.1038/s41598-021-92657-3
Scopus
Zitationen: 1
Web of Science
Zitationen: 2
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Beschleunigerphysik und Technologie (IBPT)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2021
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000134622
HGF-Programm 54.11.11 (POF IV, LK 01) Accelerator Operation, Research and Development
Erschienen in Scientific reports
Verlag Nature Research
Band 11
Heft 1
Seiten Artikel-Nr.: 13246
Vorab online veröffentlicht am 24.06.2021
Nachgewiesen in Dimensions
Web of Science
Scopus
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