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Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Altincekic, Nadide; Korn, Sophie Marianne; Qureshi, Nusrat Shahin; Dujardin, Marie; Ninot-Pedrosa, Martí; Abele, Rupert; Abi Saad, Marie Jose; Alfano, Caterina; Almeida, Fabio C. L.; Alshamleh, Islam; Amorim, Gisele Cardoso de; Anderson, Thomas K.; Anobom, Cristiane D.; Anorma, Chelsea; Bains, Jasleen Kaur; Bax, Adriaan; Blackledge, Martin; Blechar, Julius; Böckmann, Anja; ... mehr

Abstract:

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000135085
Veröffentlicht am 07.07.2021
Originalveröffentlichung
DOI: 10.3389/fmolb.2021.653148
Scopus
Zitationen: 12
Web of Science
Zitationen: 10
Dimensions
Zitationen: 19
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2296-889X
KITopen-ID: 1000135085
HGF-Programm 43.35.02 (POF IV, LK 01) Functionality of Soft Matter and Biomolecular Systems
Erschienen in Frontiers in molecular biosciences
Verlag Frontiers Media
Band 8
Seiten Art.Nr. 653148
Vorab online veröffentlicht am 10.05.2021
Schlagwörter COVID-19, SARS-CoV-2, nonstructural proteins, structural proteins, accessory proteins, intrinsically disordered region, cell-free protein synthesis, NMR spectroscopy
Nachgewiesen in Web of Science
Scopus
Dimensions
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