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Tuning Superfast Curing Thiol-Norbornene-Functionalized Gelatin Hydrogels for 3D Bioprinting

Göckler, Tobias 1; Haase, Sonja 1; Kempter, Xenia 1; Pfister, Rebecca 1; Maciel, Bruna R. 2; Grimm, Alisa 1; Molitor, Tamara 1; Willenbacher, Norbert 2; Schepers, Ute 1,3
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
2 Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)
3 Institut für Organische Chemie (IOC), Karlsruher Institut für Technologie (KIT)

Abstract:

Photocurable gelatin-based hydrogels have established themselves as powerful bioinks in tissue engineering due to their excellent biocompatibility, biodegradability, light responsiveness, thermosensitivity and bioprinting properties. While gelatin methacryloyl (GelMA) has been the gold standard for many years, thiol-ene hydrogel systems based on norbornene-functionalized gelatin (GelNB) and a thiolated crosslinker have recently gained increasing importance. In this paper, a highly reproducible water-based synthesis of GelNB is presented, avoiding the use of dimethyl sulfoxide (DMSO) as organic solvent and covering a broad range of degrees of functionalization (DoF: 20% to 97%). Mixing with thiolated gelatin (GelS) results in the superfast curing photoclick hydrogel GelNB/GelS. Its superior properties over GelMA, such as substantially reduced amounts of photoinitiator (0.03% (w/v)), superfast curing (1–2 s), higher network homogeneity, post-polymerization functionalization ability, minimal cross-reactivity with cellular components, and improved biocompatibility of hydrogel precursors and degradation products lead to increased survival of primary cells in 3D bioprinting. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000135263
Veröffentlicht am 13.07.2021
Originalveröffentlichung
DOI: 10.1002/adhm.202100206
Scopus
Zitationen: 9
Web of Science
Zitationen: 6
Dimensions
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2192-2640, 2192-2659
KITopen-ID: 1000135263
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Advanced Healthcare Materials
Verlag Wiley-VCH Verlag
Band 10
Heft 14
Seiten Art.-Nr. 2100206
Nachgewiesen in Scopus
Dimensions
Web of Science
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