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The Pyrococcus furiosus ironome is dominated by [Fe$_{4}$S$_{4}$]$^{2+}$ clusters or thioferrate-like iron depending on the availability of elemental sulfur

Vali, Shaik W.; Haja, Dominik K.; Brand, Richard A. 1; Adams, Michael W. W.; Lindahl, Paul A.
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)


Pyrococcus furiosus is a hyperthermophilic anaerobic archaeon whose metabolism depends on whether elemental sulfur is (+S$^{0}$) or is not (-S$^{0}$) included in growth medium. Under +S$^{0}$ conditions, expression of respiratory hydrogenase declines while respiratory membrane-bound sulfane reductase and the putative iron-storage protein IssA increase. Our objective was to investigate the iron content of WT and ΔIssA cells under these growth conditions using Mössbauer spectroscopy. WT-S$^{0}$ cells contained ∼1 mM Fe, with ∼85% present as two spectroscopically distinct forms of S = 0 [Fe$_{4}$S$_{4}$]$^{2+}$ clusters; the remainder was mainly high-spin Fe$^{II}$. WT+S$^{0}$ cells contained 5 to 9 mM Fe, with 75 to 90% present as magnetically ordered thioferrate-like (TFL) iron nanoparticles. TFL iron was similar to chemically defined thioferrates; both consisted of Fe$^{III}$ ions coordinated by an S$_{4}$ environment, and both exhibited strong coupling between particles causing high applied fields to have little spectral effect. At high temperatures with magnetic hyperfine interactions abolished, TFL iron exhibited two doublets overlapping those of [Fe$_{4}$S$_{4}$]$^{2+}$ clusters in -S$^{0}$ cells. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000135265
Veröffentlicht am 17.07.2021
DOI: 10.1016/j.jbc.2021.100710
Zitationen: 2
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1864-6158, 1864-6166
KITopen-ID: 1000135265
HGF-Programm 43.31.01 (POF IV, LK 01) Multifunctionality Molecular Design & Material Architecture
Erschienen in Journal of chemical biology
Verlag Springer-Verlag
Band 296
Seiten 100710
Nachgewiesen in Web of Science
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