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Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices

Ihle, Michaela; Biber, Stephanie; Schroeder, Insa S.; Blattner, Christine 1; Deniz, Miriam; Damia, Giovanna; Gottifredi, Vanesa; Wiesmüller, Lisa
1 Institut für Technik der Informationsverarbeitung (ITIV), Karlsruher Institut für Technologie (KIT)

Abstract:

Using human embryonic, adult and cancer stem cells/stem cell-like cells (SCs), we demonstrate that DNA replication speed differs in SCs and their differentiated counterparts. While SCs decelerate DNA replication, differentiated cells synthesize DNA faster and accumulate DNA damage. Notably, both replication phenotypes depend on p53 and polymerase iota (POL$_{ι}$). By exploring protein interactions and newly synthesized DNA, we show that SCs promote complex formation of p53 and POL$_{ι}$ at replication sites. Intriguingly, in SCs the translocase ZRANB3 is recruited to POL$_{ι}$ and required for slow-down of DNA replication. The known role of ZRANB3 in fork reversal suggests that the p53–POL$_{ι}$ complex mediates slow but safe bypass of replication barriers in SCs. In differentiated cells, POL$_{ι}$ localizes more transiently to sites of DNA synthesis and no longer interacts with p53 facilitating fast POL$_{ι}$-dependent DNA replication. In this alternative scenario, POL$_{ι}$ associates with the p53 target p21, which antagonizes PCNA poly-ubiquitination and, thereby potentially disfavors the recruitment of translocases. Altogether, we provide evidence for diametrically opposed DNA replication phenotypes in SCs and their differentiated counterparts putting DNA replication-based strategies in the spotlight for the creation of therapeutic opportunities targeting SCs.


Verlagsausgabe §
DOI: 10.5445/IR/1000135748
Veröffentlicht am 22.07.2021
Originalveröffentlichung
DOI: 10.1093/nar/gkab526
Scopus
Zitationen: 5
Web of Science
Zitationen: 3
Dimensions
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 21.07.2021
Sprache Englisch
Identifikator ISSN: 0305-1048, 1362-4962
KITopen-ID: 1000135748
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Nucleic acids research
Verlag Oxford University Press (OUP)
Band 49
Heft 13
Seiten 7457–7475
Vorab online veröffentlicht am 24.06.2021
Schlagwörter Genome integrity, repair and replication
Nachgewiesen in Web of Science
Dimensions
Scopus
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