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Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays

Liu, Hui-Yu 1; Kumar, Ravi 1; Zhong, Chunting 1; Gorji, Saleh 1; Paniushkina, Liliia; Masood, Ramsha; Wittel, Uwe A.; Fuchs, Harald 1; Nazarenko, Irina; Hirtz, Michael ORCID iD icon 1
1 Karlsruhe Nano Micro Facility (KNMF), Karlsruher Institut für Technologie (KIT)


Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer-associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer-associated EVs from complex biofluids (e.g., blood). Here, a novel EV-capture strategy based on dip-pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV- and cancer-specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer-associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies.

Verlagsausgabe §
DOI: 10.5445/IR/1000135936
Veröffentlicht am 29.07.2021
DOI: 10.1002/adma.202008493
Zitationen: 20
Web of Science
Zitationen: 17
Zitationen: 27
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0935-9648, 1521-4095
KITopen-ID: 1000135936
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Erschienen in Advanced materials
Verlag John Wiley and Sons
Band 33
Heft 35
Seiten Art.-Nr.: 2008493
Bemerkung zur Veröffentlichung Project IDs: 2019-021-025829 (DPN), 2020-024-029290 (DPN)
Vorab online veröffentlicht am 26.07.2021
Nachgewiesen in Web of Science
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