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Calculation of the flux density function for protein crystals from small scale settling and filtration experiments

Radel, Benjamin ORCID iD icon 1; Nguyen, Tu Hoang; Nirschl, Hermann 1
1 Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)


Development and engineering of protein crystals regarding mechanical stability and crystallizability occurs on a small scale. Later in the process chain of industrial production however, filtration properties are important to separate the crystals from mother liquor. Many protein crystals are sensitive to mechanical stress which is why it is important to know the filtration behavior early on. In this study, we analyze settling and filtration behavior of isometric, rod-like and needle shaped lysozyme and rod-like alcohol dehydrogenase (ADH) crystals on a small scale using an optical analytical centrifuge. Needle shaped lysozyme and rod-like ADH crystals show compressible material behavior. With the results from settling and filtration experiments, the flux density function is calculated and modeled which can be used to describe the whole settling and permeation process in dependency of the solids volume fraction. This is also an issue for simulations of industrial processes.

Verlagsausgabe §
DOI: 10.5445/IR/1000135960
DOI: 10.1002/aic.17378
Zitationen: 1
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0001-1541, 1547-5905
KITopen-ID: 1000135960
Erschienen in AIChE journal
Verlag John Wiley and Sons
Band 67
Heft 11
Seiten Art.-Nr.: e17378
Vorab online veröffentlicht am 20.07.2021
Nachgewiesen in Web of Science
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