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Authentication of holy basil using markers relating to a toxicology-relevant compound

Ríos-Rodríguez, Daniela 1; Sahi, Vaidurya Pratap 1; Nick, Peter 1
1 Karlsruher Institut für Technologie (KIT)


Holy Basil—Ocimum tenuiflorum—is one of the popular new “superfoods” thought to act as an antioxidant and to reduce stress and anxiety. However, it is often surrogated with other Ocimum species differing in their chemical profiles that may even pose health risks to the consumers. Moreover, even specific chemotypes of Holy Basil itself can be toxicologically relevant, because they sometimes contain the carcinogen compound methyleugenol. Using DNA barcoding based on plastidic markers, O. tenuiflorum can be differentiated from other species of Ocimum. However, this approach is still suboptimal in handling larger sample numbers and in tracing chemotypes that accumulate methyleugenol. We have, therefore, designed a trait-related DNA barcode based on the enzyme eugenol O-methyltransferase (EOMT), responsible for the synthesis of methyleugenol. We show that a multiplex PCR combining trait-related and trait-independent markers can differentiate O. tenuiflorum from other Ocimum species and identify methyleugenol chemotypes of O. tenuiflorum, even in dried material sold as mixtures.

Verlagsausgabe §
DOI: 10.5445/IR/1000136001
Veröffentlicht am 30.07.2021
DOI: 10.1007/s00217-021-03812-z
Zitationen: 6
Web of Science
Zitationen: 6
Zitationen: 7
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1438-2377, 0044-3026, 0372-9419, 0373-0174, 1431-4630, 1432-2331, 1438-2385
KITopen-ID: 1000136001
Erschienen in European Food Research and Technology
Verlag Springer-Verlag
Band 247
Seiten 2485–2497
Vorab online veröffentlicht am 10.07.2021
Nachgewiesen in Scopus
Web of Science
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