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Tackling tumour cell heterogeneity at the super-resolution level in human colorectal cancer tissue

Lang, Fabian 1; Contreras-Gerenas, María F.; Gelléri, Márton; Neumann, Jan; Kröger, Ole; Sadlo, Filip; Berniak, Krzysztof; Marx, Alexander; Cremer, Christoph; Wagenknecht, Hans-Achim 1; Allgayer, Heike
1 Institut für Organische Chemie (IOC), Karlsruher Institut für Technologie (KIT)

Abstract:

Tumour cell heterogeneity, and its early individual diagnosis, is one of the most fundamental problems in cancer diagnosis and therapy. Single molecule localisation microscopy (SMLM) resolves subcellular features but has been limited to cultured cell lines only. Since nuclear chromatin architecture and microRNAs are critical in metastasis, we introduce a first-in-field approach for quantitative SMLM-analysis of chromatin nanostructure in individual cells in resected, routine-pathology colorectal carcinoma (CRC) patient tissue sections. Chromatin density profiles proved to differ for cells in normal and carcinoma colorectal tissues. In tumour sections, nuclear size and chromatin compaction percentages were significantly different in carcinoma versus normal epithelial and other cells of colorectal tissue. SMLM analysis in nuclei from normal colorectal tissue revealed abrupt changes in chromatin density profiles at the nanoscale, features not detected by conventional widefield microscopy. SMLM for microRNAs relevant for metastasis was achieved in colorectal cancer tissue at the nuclear level. Super-resolution microscopy with quantitative image evaluation algorithms provide powerful tools to analyse chromatin nanostructure and microRNAs of individual cells from normal and tumour tissue at the nanoscale. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000136122
Veröffentlicht am 12.08.2021
Originalveröffentlichung
DOI: 10.3390/cancers13153692
Scopus
Zitationen: 4
Web of Science
Zitationen: 4
Dimensions
Zitationen: 9
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2072-6694
KITopen-ID: 1000136122
Erschienen in Cancers
Verlag MDPI
Band 13
Heft 15
Seiten 3692
Schlagwörter chromatin density; nanoscale; tumour cell heterogeneity; microRNAs; metastasis; super-resolution microscopy
Nachgewiesen in Web of Science
Scopus
Dimensions
Globale Ziele für nachhaltige Entwicklung Ziel 3 – Gesundheit und Wohlergehen
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