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Real‐Time NMR Monitoring of Spatially Segregated Enzymatic Reactions in Multilayered Hydrogel Assemblies**

Nordin, Nurdiana 1; Bordonali, Lorenzo 1; Davoodi, Hossein 1; Ratnawati, Novindi Dwi 1; Gygli, Gudrun 2; Korvink, Jan G. 1; Badilita, Vlad 1; MacKinnon, Neil 1
1 Institut für Mikrostrukturtechnik (IMT), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


Compartmentalized chemical reactions at the microscale are important in biotechnology, yet monitoring the molecular content at these small scales is challenging. To address this challenge, we integrate a compact, reconfigurable reaction cell featuring electrochemical functionality with high-resolution NMR spectroscopy. We demonstrate the operation of this system by monitoring the activity of enzymes immobilized in chemically distinct layers within a multi-layered chitosan hydrogel assembly. As a benchmark, we observed the parallel activities of urease (Urs), catalase (Cat), and glucose oxidase (GOx) by monitoring reagent and product concentrations in real-time. Simultaneous monitoring of an independent enzymatic process (Urs) together with a cooperative process (GOx + Cat) was achieved, with chemical conversion modulation of the GOx + Cat process demonstrated by varying the order in which the hydrogel was assembled.

Verlagsausgabe §
DOI: 10.5445/IR/1000136479
Veröffentlicht am 17.08.2021
DOI: 10.1002/anie.202103585
Zitationen: 3
Web of Science
Zitationen: 1
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 23.08.2021
Sprache Englisch
Identifikator ISSN: 1433-7851, 1521-3773
KITopen-ID: 1000136479
HGF-Programm 43.35.02 (POF IV, LK 01) Functionality of Soft Matter and Biomolecular Systems
Weitere HGF-Programme 43.35.01 (POF IV, LK 01) Platform for Correlative, In Situ & Operando Charakterizat.
Erschienen in Angewandte Chemie / International edition
Verlag John Wiley and Sons
Band 60
Heft 35
Seiten 19176–19182
Vorab online veröffentlicht am 16.06.2021
Nachgewiesen in Dimensions
Web of Science
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