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Genome Mining and Comparative Genome Analysis Revealed Niche-Specific Genome Expansion in Antibacterial Bacillus pumilus Strain SF-4

Iqbal, Sajid; Vollmers, John 1; Janjua, Hussnain Ahmed
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


The present study reports the isolation of antibacterial exhibiting Bacillus pumilus (B. pumilus) SF-4 from soil field. The genome of this strain SF-4 was sequenced and analyzed to acquire in-depth genomic level insight related to functional diversity, evolutionary history, and biosynthetic potential. The genome of the strain SF-4 harbor 12 Biosynthetic Gene Clusters (BGCs) including four Non-ribosomal peptide synthetases (NRPSs), two terpenes, and one each of Type III polyketide synthases (PKSs), hybrid (NRPS/PKS), lipopeptide, β-lactone, and bacteriocin clusters. Plant growth-promoting genes associated with de-nitrification, iron acquisition, phosphate solubilization, and nitrogen metabolism were also observed in the genome. Furthermore, all the available complete genomes of B. pumilus strains were used to highlight species boundaries and diverse niche adaptation strategies. Phylogenetic analyses revealed local diversification and indicate that strain SF-4 is a sister group to SAFR-032 and 150a. Pan-genome analyses of 12 targeted strains showed regions of genome plasticity which regulate function of these strains and proposed direct strain adaptations to specific habitats. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000136902
Veröffentlicht am 30.08.2021
DOI: 10.3390/genes12071060
Zitationen: 15
Web of Science
Zitationen: 14
Zitationen: 14
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2073-4425
KITopen-ID: 1000136902
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Genes
Verlag MDPI
Band 12
Heft 7
Seiten Art.-Nr.: 1060
Vorab online veröffentlicht am 12.07.2021
Schlagwörter Bacillus pumilus; whole-genome sequence; biosynthetic gene clusters (BGCs); plant growth-promoting bacteria; phylogenetic and comparative genome analysis; genome plasticity
Nachgewiesen in Dimensions
Web of Science
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