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Multi-colour fluorescence imaging of photosynthetic activity and plant stress

Lichtenthaler, H. K. 1
1 Karlsruher Institut für Technologie (KIT)


Imaging the four fluorescence bands of leaves, the red (F$_{690}$) and far-red (F$_{740}$) chlorophyll (Chl) fluorescence as well as the blue (F$_{440}$) and green (F$_{520}$) fluorescence of leaves and the corresponding fluorescence ratios is a fast and excellent nondestructive technique to detect the photosynthetic activity and capacity of leaves, of gradients over the leaf area as well as the effect of various strain and stress parameters on plants. This review primarily deals with the first and pioneering multi-colour fluorescence imaging results obtained since the mid-1990s in a cooperation with French colleagues in Strasbourg and in my laboratory in Karlsruhe. Together we introduced not only the joint imaging of the red and far-red Chl fluorescence but also of the blue and green fluorescence of leaves. The two instrumental setups composed for this purpose were (1) the Karlsruhe–Strasbourg UV-Laser Fluorescence Imaging System (Laser-FIS) and (2) the Karlsruhe Flash-Light Fluorescence Imaging System (FL-FIS). Essential results obtained with these instruments are summarized as well as the basic principles and characteristics of multi-colour fluorescence imaging. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000137003
Veröffentlicht am 23.09.2021
DOI: 10.32615/ps.2021.020
Zitationen: 10
Web of Science
Zitationen: 10
Zitationen: 10
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0300-3604, 1573-9058
KITopen-ID: 1000137003
Erschienen in Photosynthetica
Verlag Academy of Sciences of the Czech Republic, Institute of Experimental Botany
Band 59
Heft S1
Seiten 364-380
Schlagwörter chlorophyll fluorescence decrease ratio, chlorophyll fluorescence induction kinetics, fluorescence ratio images, Kautsky effect
Nachgewiesen in Web of Science
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