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Formulation of DNA Nanocomposites: Towards Functional Materials for Protein Expression

Schipperges, Alessa 1; Hu, Yong; Moench, Svenja ORCID iD icon 1; Weigel, Simone 1; Reith, Johannes 1; Ordoñez-Rueda, Diana; Rabe, Kersten S. ORCID iD icon 1; Niemeyer, Christof M. ORCID iD icon 1
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

Abstract:

DNA hydrogels are an emerging class of materials that hold great promise for numerous biotechnological applications, ranging from tissue engineering to targeted drug delivery and cell-free protein synthesis (CFPS). In addition to the molecular programmability of DNA that can be used to instruct biological systems, the formulation of DNA materials, e.g., as bulk hydrogels or microgels, is also relevant for specific applications. To advance the state of knowledge in this research area, the present work explores the scope of a recently developed class of complex DNA nanocomposites, synthesized by RCA polymerization of DNA-functionalized silica nanoparticles (SiNPs) and carbon nanotubes (CNTs). SiNP/CNT-DNA composites were produced as bulk materials and microgels which contained a plasmid with transcribable genetic information for a fluorescent marker protein. Using confocal microscopy and flow cytometry, we found that the materials are very efficiently taken up by various eukaryotic cell lines, which were able to continue dividing while the ingested material was evenly distributed to the daughter cells. However, no expression of the encoded protein occurred within the cells. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000137378
Veröffentlicht am 14.09.2021
Originalveröffentlichung
DOI: 10.3390/polym13152395
Scopus
Zitationen: 5
Web of Science
Zitationen: 5
Dimensions
Zitationen: 6
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2073-4360
KITopen-ID: 1000137378
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Polymers
Verlag MDPI
Band 13
Heft 15
Seiten Art.-Nr.: 2395
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 21.07.2021
Schlagwörter hydrogel; protein expression; nanoparticles; DNA nanotechnology; cellular uptake; cell free protein synthesis; composite materials
Nachgewiesen in Web of Science
Scopus
Dimensions
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