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Global Transcriptome Profile of the Oleaginous Yeast Saitozyma podzolica DSM 27192 Cultivated in Glucose and Xylose

Aliyu, Habibu 1; Gorte, Olga 1; Neumann, Anke 1; Ochsenreither, Katrin ORCID iD icon 1
1 Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Unlike conventional yeasts, several oleaginous yeasts, including Saitozyma podzolica DSM 27192, possess the innate ability to grow and produce biochemicals from plant-derived lignocellulosic components such as hexose and pentose sugars. To elucidate the genetic basis of S. podzolica growth and lipid production on glucose and xylose, we performed comparative temporal transcriptome analysis using RNA-seq method. Approximately 3.4 and 22.2% of the 10,670 expressed genes were differentially (FDR < 0.05, and log2FC > 1.5) expressed under batch and fed batch modes, respectively. Our analysis revealed that a higher number of sugar transporter genes were significantly overrepresented in xylose relative to glucose-grown cultures. Given the low homology between proteins encoded by most of these genes and those of the well-characterised transporters, it is plausible to conclude that S. podzolica possesses a cache of putatively novel sugar transporters. The analysis also suggests that S. podzolica potentially channels carbon flux from xylose via both the non-oxidative pentose phosphate and potentially via the first steps of the Weimberg pathways to yield xylonic acid. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000137410
Veröffentlicht am 15.09.2021
DOI: 10.3390/jof7090758
Zitationen: 4
Web of Science
Zitationen: 3
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 09.2021
Sprache Englisch
Identifikator ISSN: 2309-608X
KITopen-ID: 1000137410
Erschienen in Journal of Fungi
Verlag MDPI
Band 7
Heft 9
Seiten Art.-Nr.: 758
Bemerkung zur Veröffentlichung This article belongs to the Section Fungal Genomics, Genetics and Molecular Biology
Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 15.09.2021
Schlagwörter glucose; microbial lipid; oleaginous yeast; RNA-seq; Saitozyma podzolica DSM 27192; sugar transporters; Weimberg pathway; xylose
Nachgewiesen in Dimensions
Web of Science
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