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Rapid quantitative assays for glucose-6-phosphate dehydrogenase (G6PD) and hemoglobin combined on a capillary-driven microfluidic chip

Rocca, Marco 1; Temiz, Yuksel; Salva, Marie L. 1; Castonguay, Samuel; Gervais, Thomas; Niemeyer, Christof M. ORCID iD icon 1; Delamarche, Emmanuel
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)


Rapid tests for glucose-6-phosphate dehydrogenase (G6PD) are extremely important for determining G6PD deficiency, a widespread metabolic disorder which triggers hemolytic anemia in response to primaquine and tafenoquine medication, the most effective drugs for the radical cure of malaria caused by Plasmodium parasites. Current point-of-care diagnostic devices for G6PD are either qualitative, do not normalize G6PD activity to the hemoglobin concentration, or are very expensive. In this work we developed a capillary-driven microfluidic chip to perform a quantitative G6PD test and a hemoglobin measurement within 2 minutes and using less than 2 μL of sample. We used a powerful microfluidic module to integrate and resuspend locally the reagents needed for the G6PD assay and controls. We also developed a theoretical model that successfully predicts the enzymatic reactions on-chip, guides on-chip reagent spotting and allows efficient integration of multiple assays in miniaturized formats with only a few nanograms of reagents.

Verlagsausgabe §
DOI: 10.5445/IR/1000137437
Veröffentlicht am 15.09.2021
DOI: 10.1039/d1lc00354b
Zitationen: 5
Web of Science
Zitationen: 4
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1473-0197, 1473-0189
KITopen-ID: 1000137437
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Lab on a chip
Verlag Royal Society of Chemistry (RSC)
Band 21
Heft 18
Seiten 3573–3582
Vorab online veröffentlicht am 27.07.2021
Nachgewiesen in Scopus
Web of Science
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