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Effect of HIT Components on the Development of Breast Cancer Cells

Chen, L.-Y.; Apte, Gurunath ORCID iD icon 1; Lindenbauer, A.; Frant, M.; Nguyen, T.-H.
1 Karlsruhe Nano Micro Facility (KNMF), Karlsruher Institut für Technologie (KIT)


Cancer cells circulating in blood vessels activate platelets, forming a cancer cell encircling platelet cloak which facilitates cancer metastasis. Heparin (H) is frequently used as an anticoagulant in cancer patients but up to 5% of patients have a side effect, heparin-induced thrombocytopenia (HIT) that can be life-threatening. HIT is developed due to a complex interaction among multiple components including heparin, platelet factor 4 (PF4), HIT antibodies, and platelets. However, available information regarding the effect of HIT components on cancers is limited. Here, we investigated the effect of these materials on the mechanical property of breast cancer cells using atomic force microscopy (AFM) while cell spreading was quantified by confocal laser scanning microscopy (CLSM), and cell proliferation rate was determined. Over time, we found a clear effect of each component on cell elasticity and cell spreading. In the absence of platelets, HIT antibodies inhibited cell proliferation but they promoted cell proliferation in the presence of platelets. Our results indicate that HIT complexes influenced the development of breast cancer cells.

Verlagsausgabe §
DOI: 10.5445/IR/1000137473
Veröffentlicht am 01.10.2021
DOI: 10.3390/life11080832
Zitationen: 3
Web of Science
Zitationen: 2
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2075-1729
KITopen-ID: 1000137473
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Erschienen in Life
Verlag MDPI
Band 11
Heft 8
Seiten Art.-Nr.: 832
Nachgewiesen in Web of Science
Globale Ziele für nachhaltige Entwicklung Ziel 3 – Gesundheit und Wohlergehen
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