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A fast and reliable method for monitoring genomic instability in the model organism Caenorhabditis elegans

Nicolai, M. M.; Witt, B.; Hartwig, Andrea 1,2; Schwerdtle, T.; Bornhorst, J.
1 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)
2 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)


The identification of genotoxic agents and their potential for genotoxic alterations in an organism is crucial for risk assessment and approval procedures of the chemical and pharmaceutical industry. Classically, testing strategies for DNA or chromosomal damage focus on in vitro and in vivo (mainly rodent) investigations. In cell culture systems, the alkaline unwinding (AU) assay is one of the well-established methods for detecting the percentage of double-stranded DNA (dsDNA). By establishing a reliable lysis protocol, and further optimization of the AU assay for the model organism Caenorhabditis elegans (C. elegans), we provided a new tool for genotoxicity testing in the niche between in vitro and rodent experiments. The method is intended to complement existing testing strategies by a multicellular organism, which allows higher predictability of genotoxic potential compared to in vitro cell line or bacterial investigations, before utilizing in vivo (rodent) investigations. This also allows working within the 3R concept (reduction, refinement, and replacement of animal experiments), by reducing and possibly replacing animal testing. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000137482
Veröffentlicht am 21.09.2021
DOI: 10.1007/s00204-021-03144-7
Zitationen: 2
Web of Science
Zitationen: 2
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0340-5761, 0003-9446, 0370-8497, 1432-0738
KITopen-ID: 1000137482
Erschienen in Archives of Toxicology
Verlag Springer
Band 95
Seiten 3417–3424
Vorab online veröffentlicht am 30.08.2021
Nachgewiesen in Dimensions
Web of Science
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