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Effect of Oxygen Contamination on Propionate and Caproate Formation in Anaerobic Fermentation

Baleeiro, F. C. F. 1; Ardila, M. S. 1; Kleinsteuber, S.; Sträuber, H.
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)


Mixed microbial cultures have become a preferred choice of biocatalyst for chain elongation systems due to their ability to convert complex substrates into medium-chain carboxylates. However, the complexity of the effects of process parameters on the microbial metabolic networks is a drawback that makes the task of optimizing product selectivity challenging. Here, we studied the effects of small air contaminations on the microbial community dynamics and the product formation in anaerobic bioreactors fed with lactate, acetate and H$_{2}$/CO$_{2}$. Two stirred tank reactors and two bubble column reactors were operated with H$_{2}$/CO$_{2}$ gas recirculation for 139 and 116 days, respectively, at pH 6.0 and 32°C with a hydraulic retention time of 14 days. One reactor of each type had periods with air contamination (between 97 ± 28 and 474 ± 33 mL O$_{2}$ L$^{-1}$ d$^{-1}$, lasting from 4 to 32 days), while the control reactors were kept anoxic. During air contamination, production of n-caproate and CH$_{4}$ was strongly inhibited, whereas no clear effect on n-butyrate production was observed. In a period with detectable O$_{2}$ concentrations that went up to 18%, facultative anaerobes of the genus Rummeliibacillus became predominant and only n-butyrate was produced. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000138668
Veröffentlicht am 06.10.2021
DOI: 10.3389/fbioe.2021.725443
Zitationen: 6
Zitationen: 12
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000138668
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 9
Seiten Art. Nr.: 725443
Vorab online veröffentlicht am 10.09.2021
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