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Fluorescent Nanozeolite Receptors for the Highly Selective and Sensitive Detection of Neurotransmitters in Water and Biofluids

Grimm, L. M. 1; Sinn, S. 1; Krstić, M. 1; D’Este, E.; Sonntag, I.; Prasetyanto, E. A.; Kuner, T. 2; Wenzel, W. 1; De Cola, L. 1; Biedermann, F. 1,2
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
2 Heidelberg Karlsruhe Strategic Partnership (HEiKA), Karlsruher Institut für Technologie (KIT)

Abstract:

The design and preparation of synthetic binders (SBs) applicable for small biomolecule sensing in aqueous media remains very challenging. SBs designed by the lock-and-key principle can be selective for their target analyte but usually show an insufficient binding strength in water. In contrast, SBs based on symmetric macrocycles with a hydrophobic cavity can display high binding affinities but generally suffer from indiscriminate binding of many analytes. Herein, a completely new and modular receptor design strategy based on microporous hybrid materials is presented yielding zeolite-based artificial receptors (ZARs) which reversibly bind the neurotransmitters serotonin and dopamine with unprecedented affinity and selectivity even in saline biofluids. ZARs are thought to uniquely exploit both the non-classical hydrophobic effect and direct non-covalent recognition motifs, which is supported by in-depth photophysical, and calorimetric experiments combined with full atomistic modeling. ZARs are thermally and chemically robust and can be readily prepared at gram scales. Their applicability for the label-free monitoring of important enzymatic reactions, for (two-photon) fluorescence imaging, and for high-throughput diagnostics in biofluids is demonstrated. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000138689
Veröffentlicht am 06.10.2021
Originalveröffentlichung
DOI: 10.1002/adma.202104614
Scopus
Zitationen: 16
Web of Science
Zitationen: 15
Dimensions
Zitationen: 15
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0935-9648, 1521-4095
KITopen-ID: 1000138689
HGF-Programm 43.32.01 (POF IV, LK 01) Molecular Materials Basis for Optics & Photonics
Erschienen in Advanced Materials
Verlag John Wiley and Sons
Band 33
Heft 49
Seiten Art.-Nr.: 2104614
Nachgewiesen in Dimensions
Web of Science
Scopus
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