The draft genome sequence of Scheffersomyces segobiensis strain DSM 27193, a yeast capable of producing palmitoleic acid-rich lipids
Zhou, Xinhai; Xu, Lijie; Zhou, Dawei; Zhou, Jie; Xin, Fengxue; Zhang, Wenming; Qian, Xiujuan; Dong, Weiliang; Jiang, Min; Ochsenreither, Katrin
1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)
11 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)
Abstract (englisch):
In this study, the draft genome of the recently isolated oleaginous yeast Schefersomyces segobiensis DSM 27193, which
can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about
POA-rich lipid production by Schefersomyces segobiensis so far. The ITS region analysis indicated that strain DSM 27193
is closely related to Pichia segobiensis and Schefersomyces stipitis. The size of the assembled draft genome of strain DSM
27193 is 14.8 Mb, containing 5477 encoded protein sequences with a G+C content of 40.83%. Among the annotated genes,
two stearoyl-CoA desaturases encoded by ole1 and ole2 were identifed which are potentially involved in POA accumulation.
Further analysis of POA-rich lipid synthesis pathway genes in S. segobiensis DSM 27193 will provide additional insights
for POA artifcial synthesis through metabolic engineering
can accumulate high content of palmitoleic acid (POA), was sequenced and analyzed. Only few studies have reported about
POA-rich lipid production by Schefersomyces segobiensis so far. The ITS region analysis indicated that strain DSM 27193
is closely related to Pichia segobiensis and Schefersomyces stipitis. The size of the assembled draft genome of strain DSM
27193 is 14.8 Mb, containing 5477 encoded protein sequences with a G+C content of 40.83%. Among the annotated genes,
two stearoyl-CoA desaturases encoded by ole1 and ole2 were identifed which are potentially involved in POA accumulation.
Further analysis of POA-rich lipid synthesis pathway genes in S. segobiensis DSM 27193 will provide additional insights
for POA artifcial synthesis through metabolic engineering
| Zugehörige Institution(en) am KIT | Institut für Bio- und Lebensmitteltechnik (BLT) |
| Publikationstyp | Zeitschriftenaufsatz |
| Publikationsmonat/-jahr | 11.2021 |
| Sprache | Englisch |
| Identifikator | ISSN: 2190-572X, 2190-5738 KITopen-ID: 1000139683 |
| Erschienen in | 3 Biotech |
| Verlag | Springer-Verlag |
| Band | 11 |
| Heft | 11 |
| Seiten | Article no: 488 |
| Vorab online veröffentlicht am | 03.11.2021 |
| Schlagwörter | Draft genome; Schefersomyces segobiensis; Palmitoleic acid; Microbial lipid; Stearoyl-CoA desaturases |
| Nachgewiesen in | Dimensions Scopus Web of Science |