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Dissecting the membrane-microtubule sensor in grapevine defence

Guan, Pingyin; Shi, Wenjing ORCID iD icon; Riemann, Michael; Nick, Peter


Specific populations of plant microtubules cooperate with the plasma membrane to sense and process abiotic stress signals, such as cold stress. The current study derived from the question, to what extent this perception system is active in biotic stress signalling. The experimental system consisted of grapevine cell lines, where microtubules or actin filaments are visualised by GFP, such that their response became visible in vivo. We used the bacterial elicitors harpin (inducing cell-death related defence), or flg22 (inducing basal immunity) in combination with modulators of membrane fluidity, or microtubules. We show that DMSO, a membrane rigidifier, can cause microtubule bundling and trigger defence responses, including activation of phytoalexin transcripts. However, DMSO inhibited the gene expression in response to harpin, while promoting the gene expression in response to flg22. Treatment with DMSO also rendered microtubules more persistent to harpin. Paradoxically, Benzylalcohol (BA), a membrane fluidiser, acted in the same way as DMSO. Neither GdCl3, nor diphenylene iodonium were able to block the inhibitory effect of membrane rigidification on harpin-induced gene expression. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000140888
Veröffentlicht am 08.12.2021
DOI: 10.1038/s41438-021-00703-y
Zitationen: 4
Web of Science
Zitationen: 4
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2052-7276, 2662-6810
KITopen-ID: 1000140888
Erschienen in Horticulture Research
Verlag Springer Nature [academic journals on]
Band 8
Heft 1
Seiten 260
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Nachgewiesen in Dimensions
Web of Science
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