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The DNA-dependent protease AtWSS1A suppresses persistent double strand break formation during replication

Hacker, Leonie; Capdeville, Niklas; Feller, Laura; Enderle-Kukla, Janina; Dorn, Annika; Puchta, Holger


The protease WSS1A is an important factor in the repair of DNA-protein crosslinks in plants.
Here we show that the loss of WSS1A leads to a reduction of 45S rDNA repeats and chromosomal fragmentation in Arabidopsis. Moreover, in the absence of any factor of the RTR (RECQ4A/TOP3α/RMI1/2) complex, which is involved in the dissolution of DNA replication intermediates, WSS1A becomes essential for viability.
If WSS1A loss is combined with loss of the classical (c) or alternative (a) nonhomologous end joining (NHEJ) pathways of double-strand break (DSB) repair, the resulting mutants show proliferation defects and enhanced chromosome fragmentation, which is especially aggravated in the absence of aNHEJ. This indicates that WSS1A is involved either in the suppression of DSB formation or in DSB repair itself. To test the latter we induced DSB by CRISPR/Cas9 at different loci in wild-type and mutant cells and analyzed their repair by deep sequencing. However, no change in the quality of the repair events and only a slight increase in their quantity was found.
Thus, by removing complex DNA-protein structures, WSS1A seems to be required for the repair of replication intermediates which would otherwise be resolved into persistent DSB leading to genome instability.

Verlagsausgabe §
DOI: 10.5445/IR/1000140922
Veröffentlicht am 08.12.2021
DOI: 10.1111/nph.17848
Zitationen: 1
Web of Science
Zitationen: 1
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0028-646X, 1469-8137
KITopen-ID: 1000140922
Erschienen in New Phytologist
Verlag John Wiley and Sons
Band 233
Heft 3
Seiten 1172-1187
Projektinformation DFG, DFG EIN, PU 137/22-1
Nachgewiesen in Scopus
Web of Science
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