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The Nma1 protein promotes long distance transport mediated by early endosomes in Ustilago maydis

Schneider, Karina; Farr, Theresa; Pinter, Niko; Schmitt, Kerstin; Valerius, Oliver; Braus, Gerhard H.; Kämper, Jörg


Early endosomes (EEs) are part of the endocytic transport pathway and resemble the earliest class of transport vesicles between the internalization of extracellular material, their cellular distribution or vacuolar degradation. In filamentous fungi, EEs fulfill important functions in long distance transport of cargoes as mRNAs, ribosomes, and peroxisomes. Formation and maturation of early endosomes is controlled by the specific membrane-bound Rab-GTPase Rab5 and tethering complexes as CORVET (class C core vacuole/endosome tethering). In the basidiomycete Ustilago maydis, Rab5a is the prominent GTPase to recruit CORVET to EEs; in rab5a deletion strains, this function is maintained by the second EE-associated GTPase Rab5b. The tethering- and core-subunits of CORVET are essential, buttressing a central role for EE transport in U. maydis. The function of EEs in long distance transport is supported by the Nma1 protein that interacts with the Vps3 subunit of CORVET. The interaction stabilizes the binding of Vps3 to the CORVET core complex that is recruited to Rab5a via Vps8. Deletion of nma1 leads to a significantly reduced number of EEs, and an increased conversion rate of EEs to late endosomes. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000141123
Veröffentlicht am 15.12.2021
DOI: 10.1111/mmi.14851
Zitationen: 1
Web of Science
Zitationen: 1
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Karlsruher Institut für Technologie (KIT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 0950-382X, 1365-2958
KITopen-ID: 1000141123
Erschienen in Molecular Microbiology
Verlag John Wiley and Sons
Band 117
Heft 2
Seiten 334-352
Vorab online veröffentlicht am 24.11.2021
Nachgewiesen in Dimensions
Web of Science
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