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pH-Induced Modulation of Vibrio fischeri Population Life Cycle

Silva, Ana Rita; Sousa, Cláudia; Exner, Daniela; Schwaiger, Ruth; Alves, Maria Madalena; Petrovykh, Dmitri Y.; Pereira, Luciana

Abstract:

Commonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000141356
Veröffentlicht am 18.12.2021
Originalveröffentlichung
DOI: 10.3390/chemosensors9100283
Scopus
Zitationen: 3
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Materialien – Computational Materials Science (IAM-CMS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 2227-9040
KITopen-ID: 1000141356
Erschienen in Chemosensors
Verlag MDPI
Band 9
Heft 10
Seiten 283
Vorab online veröffentlicht am 05.10.2021
Schlagwörter Vibrio fischeri; bioluminescence; cell-surface charge; scanning electron microscopy; helium-ion microscopy; zeta potential; cell morphology; cell-size distribution; environmental stress
Nachgewiesen in Dimensions
Scopus
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