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Resolution enhancement of low-NA objectives in confocal fluorescence microscopy by diffractive lens arrays

Li, Zheng; Taphanel, Miro; Längle, Thomas; Beyerer, Jürgen


In traditional confocal microscopes, high resolution and large fields of view are difficult to achieve simultaneously by microscope objectives, which limit the space-bandwidth products of the systems. With the proposed diffractive lens arrays, an objective with a low numerical aperture can have much higher resolution while maintaining its large field of view. Application of the diffractive lens arrays in fluorescence microscopy is presented in this work. The resolution enhancement is demonstrated by experiments with the fluorescent beads.

DOI: 10.1117/12.2603633
Zitationen: 1
Zugehörige Institution(en) am KIT Fraunhofer-Institut für Optronik, Systemtechnik und Bildauswertung (IOSB)
Institut für Anthropomatik und Robotik (IAR)
Publikationstyp Proceedingsbeitrag
Publikationsdatum 06.12.2021
Sprache Englisch
Identifikator KITopen-ID: 1000141651
Erschienen in Proceedings of the International Optical Design Conference 2021. Ed.: P.P. Clark
Veranstaltung International Optical Design Conference (IODC 2021), Online, 27.06.2021 – 01.07.2021
Verlag SPIE
Seiten 126 – 131
Serie Proceedings of SPIE ; 12078
Vorab online veröffentlicht am 19.11.2021
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