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Simultaneous analyte indicator binding assay (SBA) for the monitoring of reversible host–guest complexation kinetics

Miskolczy, Zsombor; Megyesi, Mónika; Sinn, Stephan; Biedermann, Frank; Biczók, László


Very little information is available on the kinetics of the self-assembly and dissociation of optically silent building blocks despite the importance of such data in the rational design of tailor-made host–guest systems. We introduce here a novel time-resolved method that enables the simultaneous determination of complex formation and complex dissociation rate constants for inclusion-type host–guest complexes. The simultaneous analyte indicator binding assay (SBA) gives also direct access to binding affinities, thus largely simplifying the experimental procedure for a full kinetic and thermodynamic characterisation of host–guest systems.

Verlagsausgabe §
DOI: 10.5445/IR/1000141667
Veröffentlicht am 08.01.2022
DOI: 10.1039/D1CC04888K
Zitationen: 5
Web of Science
Zitationen: 5
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2021
Sprache Englisch
Identifikator ISSN: 1359-7345, 1364-548X
KITopen-ID: 1000141667
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Erschienen in Chemical communications
Verlag Royal Society of Chemistry (RSC)
Band 57
Heft 94
Seiten 12663–12666
Nachgewiesen in Web of Science
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