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Immobilization Strategies for Compartmentalized Reactions in Flow Reactors

Martin Peng, Christof M. Niemeyer, Kersten S. Rabe


Keywords: 3D printing, directed evolution, enzymes, flow catalysis, hydrogels

The compartmentalization of chemical reactions is a basic principle in nature, which can be implemented in technical processes by performing reaction cascades with physically separated enzymes. For the development of novel approaches in biocatalysis this principle is a major source for innovations and is therefore mimicked in several ways. The immobilization of biocatalysts in a fluidic setup is one way to achieve compartmentalization and thus precise control over artificial reaction cascades. Many established state-of-the-art technologies to arrange enzymes for sequential reactions require chemical modifications of the target enzymes, which can negatively influence the activities or specificities of the immobilized enzymes. We recently demonstrated the encapsulation of unmodified thermostable enzymes in a 3D printed, agarose-based thermoreversible hydrogel [1,2] and the site-selective immobilization of enzymes on beads.[3]

Figure 1. Schematic manufacturing process for encapsulation of unmodified enzymes in 3D printed hydrogel structures.

Both immobilization strategies allow implementation in continuous flow systems and have been successfully utilized for multi-step sequential biotransformations. ... mehr

Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Poster
Publikationsmonat/-jahr 07.2021
Sprache Englisch
Identifikator KITopen-ID: 1000141805
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Veranstaltung Biotrans 2021 Graz (2021), Online, 19.07.2021 – 22.07.2021
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