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Immobilization of β-Galactosidase by Encapsulation of Enzyme-Conjugated Polymer Nanoparticles Inside Hydrogel Microparticles

Suvarli, N. ORCID iD icon; Wenger, L. ORCID iD icon; Serra, C.; Perner-Nochta, I.; Hubbuch, J. ORCID iD icon; Wörner, M.

Abstract:

Increasing the shelf life of enzymes and making them reusable is a prominent topic in biotechnology. The encapsulation inside hydrogel microparticles (HMPs) can enhance the enzyme’s stability by preserving its native conformation and facilitating continuous biocatalytic processes and enzyme recovery. In this study, we present a method to immobilize β-galactosidase by, first, conjugating the enzyme onto the surface of polymer nanoparticles, and then encapsulating these enzyme-conjugated nanoparticles (ENPs) inside HMPs using microfluidic device paired with UV-LEDs. Polymer nanoparticles act as anchors for enzyme molecules, potentially preventing their leaching through the hydrogel network especially during swelling. The affinity binding (through streptavidin-biotin interaction) was used as an immobilization technique of β-galactosidase on the surface of polymer nanoparticles. The hydrogel microparticles of roughly 400 μm in size (swollen state) containing unbound enzyme and ENPs were produced. The effects of encapsulation and storage in different conditions were evaluated. It was discovered that the encapsulation in acrylamide (AcAm) microparticles caused an almost complete loss of enzymatic activity. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000142853
Veröffentlicht am 10.02.2022
Originalveröffentlichung
DOI: 10.3389/fbioe.2021.818053
Scopus
Zitationen: 3
Web of Science
Zitationen: 2
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000142853
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 9
Seiten Art.-Nr.: 818053
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 13.01.2022
Nachgewiesen in Scopus
Dimensions
Web of Science
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