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FluidFM-Based Fabrication of Nanopatterns: Promising Surfaces for Platelet Storage Application

Apte, Gurunath ORCID iD icon 1; Hirtz, Michael ORCID iD icon 1; Nguyen, Thi-Huong
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)


Platelets are cell fragments from megakaryocytes devoid of the cell nucleus. They are highly sensitive and easily activated by nonphysiological surfaces. Activated platelets have an intrinsic mechanism to release various proteins that participate in multiple pathways, initiating the platelet activation cascade. Surface-induced platelet activation is a challenge encountered during platelet storage, which eventually leads to aggregation of platelets and can thereby result in the degradation of the platelet concentrates. We have previously reported that surface-induced platelet activation can be minimized by either modifying their contact surfaces with polymers or introducing nanogroove patterns underneath the platelets. Here, we investigated the response of platelets to various nanotopographical surfaces printed using fluidic force microscopy (FluidFM). We found that the hemispherical array (grid) and hexagonal tile (hive) structures caused a reduction of surface stiffness, which leads to an inhibition of platelet adhesion. Our results reveal that nanopatterns enable the inhibition of platelet activation on surfaces, thus implying that development in nanotexturing of storage bags can extend the lifetime of platelet concentrates.

Postprint §
DOI: 10.5445/IR/1000146674
Veröffentlicht am 21.05.2023
DOI: 10.1021/acsami.2c03459
Zitationen: 1
Web of Science
Zitationen: 1
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1944-8244, 1944-8252
KITopen-ID: 1000146674
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Erschienen in ACS Applied Materials & Interfaces
Verlag American Chemical Society (ACS)
Band 14
Heft 21
Seiten 24133–24143
Vorab online veröffentlicht am 20.05.2022
Schlagwörter Project-ID: 2020-023-028470 (DPN)
Nachgewiesen in Scopus
Web of Science
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