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The Minus-End-Directed Kinesin OsDLK Shuttles to the Nucleus and Modulates the Expression of Cold-Box Factor 4

Xu, Xiaolu 1; Hummel, Sabine; Harter, Klaus; Kolukisaoglu, Üner; Riemann, Michael 2; Nick, Peter 2
1 Karlsruher Institut für Technologie (KIT)
2 Botanisches Institut (BOTANIK), Karlsruher Institut für Technologie (KIT)

Abstract:

The transition to terrestrial plants was accompanied by a progressive loss of microtubule minus-end-directed dynein motors. Instead, the minus-end-directed class-XIV kinesins expanded considerably, likely related to novel functions. One of these motors, OsDLK (Dual Localisation Kinesin from rice), decorates cortical microtubules but moves into the nucleus in response to cold stress. This analysis of loss-of-function mutants in rice indicates that OsDLK participates in cell elongation during development. Since OsDLK harbours both a nuclear localisation signal and a putative leucin zipper, we asked whether the cold-induced import of OsDLK into the nucleus might correlate with specific DNA binding. Conducting a DPI-ELISA screen with recombinant OsDLKT (lacking the motor domain), we identified the Opaque2 motif as the most promising candidate. This motif is present in the promoter of NtAvr9/Cf9, the tobacco homologue of Cold-Box Factor 4, a transcription factor involved in cold adaptation. A comparative study revealed that the cold-induced accumulation of NtAvr9/Cfp9 was specifically quelled in transgenic BY−2 cells overexpressing OsDLK-GFP. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000148546
Veröffentlicht am 13.07.2022
Originalveröffentlichung
DOI: 10.3390/ijms23116291
Scopus
Zitationen: 3
Web of Science
Zitationen: 2
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1422-0067
KITopen-ID: 1000148546
Erschienen in International Journal of Molecular Sciences
Verlag MDPI
Band 23
Heft 11
Seiten Art.Nr. 6291
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 03.06.2022
Nachgewiesen in Scopus
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Web of Science
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