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Functionalization of brush-like polymer modified surfaces by scanning probe lithography for biological screening applications

Yang, Bingquan 1
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

In this dissertation, different surface modification techniques are employed in combination with microchannel cantilever spotting (µCS) to design different biochips for bioscreening and biomedical applications. For these purposes, glass slides are modified with different functional moieties, e.g., self-assembled monolayer (SAM) of alkyne and polymer brushes bearing clickable reactive groups fabricated by “grafting to” or “grafting from” approaches. Furthermore, different inks are patterned to the chips via µCS, and different ink-substrate combinations (click reactions in between them) are demonstrated, as well as the bio-screening and bio-detection are evaluated by fluorescent imaging.
To easily and conveniently evaluate the binding efficiency of cyclooctyne/azide, different derivatives of DBCO/BCN (dibenzocyclooctyne/bicyclononye) linked to either a fluorophore or a biotin-moiety are patterned on azide-bearing antifouling polymer brushes (AAPBs) via µCS. The AAPBs are composed of two building blocks, an antifouling bottom block, which can avoid unspecific protein contamination during incubation without any advancing blocking steps; and an azide functionalized top block, which can bind to alkynes via strain-promoted alkyne-azide cycloaddition (SPAAC) reactions. ... mehr


Volltext §
DOI: 10.5445/IR/1000149735
Veröffentlicht am 21.09.2022
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Anorganische Chemie (AOC)
Institut für Nanotechnologie (INT)
Publikationstyp Hochschulschrift
Publikationsdatum 21.09.2022
Sprache Englisch
Identifikator KITopen-ID: 1000149735
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Verlag Karlsruher Institut für Technologie (KIT)
Umfang XI, 131 S.
Art der Arbeit Dissertation
Fakultät Fakultät für Chemie und Biowissenschaften (CHEM-BIO)
Institut Institut für Nanotechnologie (INT)
Prüfungsdatum 19.07.2022
Schlagwörter Bioscreening, polymer brush, click chemistry
Referent/Betreuer Hirtz, Michael
Powell, Annie K.
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