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A rapid, simple, and reliable assay to authenticate Peruvian kiwicha (A. caudatus) for food applications

Kanbar, Adnan 1; Beisel, Julia 1; Wetters, Sascha 1; Gutierrez, Meylin Terrel; Graeff-Hönninger, Simone; Nick, Peter 1
1 Botanisches Institut (BOTANIK), Karlsruher Institut für Technologie (KIT)

Abstract:

Amaranth has acquired great economic impact as functional food, with species originating from Mexico dominating global trade. In contrast, the Peruvian A. caudatus (kiwicha) has been vastly neglected, although it is endowed with very promising nutritive traits. Morphological plasticity and taxonomic ambiguities render authentication of Amaranth difficult, such that the identity of commercial samples is often unclear. To safeguard the authenticity of kiwicha and, thus, consumer safety, we characterised a germplasm collection of 84 Amaranth accessions on both, the morphological and the genetic level. We show that kiwicha can be delineated phenotypically from other species by its late flowering, taller posture, and lower grain yields. Instead, flower and seed color, often used as proxy for identity, do not qualify as taxonomic markers. Using the plastidic barcoding marker psbA-trnH igs we were able to identify a specific Single Nucleotide Polymorphism (SNP) that separated kiwicha from all other species of Amaranth. This allowed us to develop a sequencing-free authentication assay using an Amplified Refractory Mutation System (ARMS) strategy. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000149812
Veröffentlicht am 15.08.2022
Originalveröffentlichung
DOI: 10.1007/s00217-022-04089-6
Scopus
Zitationen: 5
Web of Science
Zitationen: 4
Dimensions
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1438-2377, 0044-3026, 0372-9419, 0373-0174, 1431-4630, 1432-2331, 1438-2385
KITopen-ID: 1000149812
Erschienen in European Food Research and Technology
Verlag Springer-Verlag
Band 248
Heft 11
Seiten 2779–2797
Vorab online veröffentlicht am 23.07.2022
Nachgewiesen in Dimensions
Web of Science
Scopus
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