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Linker Engineering of Ligand‐Decorated DNA Origami Nanostructures Affects Biological Activity

Domínguez, Carmen M. 1; García-Chamé, Miguel 1; Müller, Ulrike; Kraus, Andreas; Gordiyenko, Klavdiya 1; Itani, Ahmad 1; Haschke, Heiko; Lanzerstorfer, Peter; Rabe, Kersten S. ORCID iD icon 1; Niemeyer, Christof M. ORCID iD icon 1
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

Abstract:

News from an old acquaintance: The streptavidin (STV)-biotin binding system is frequently used for the decoration of DNA origami nanostructures (DON) to study biological systems. Here, a surprisingly high dynamic of the STV/DON interaction is reported, which is affected by the structure of the DNA linker system. Analysis of different mono- or bi-dentate linker architectures on DON with a novel high-speed atomic force microscope (HS-AFM) enabling acquisition times as short as 50 ms per frame gave detailed insights into the dynamics of the DON/STV interaction, revealing dwell times in the sub-100 millisecond range. The linker systems are also used to present biotinylated epidermal growth factor on DON to study the activation of the epidermal growth factor receptor signaling cascade in HeLa cells. The studies confirm that cellular activation correlated with the binding properties of linker-specific STV/DON interactions observed by HS-AFM. This work sheds more light on the commonly used STV/DON system and will help to further standardize the use of DNA nanostructures for the study of biological processes.


Verlagsausgabe §
DOI: 10.5445/IR/1000150270
Veröffentlicht am 31.08.2022
Originalveröffentlichung
DOI: 10.1002/smll.202202704
Scopus
Zitationen: 6
Web of Science
Zitationen: 6
Dimensions
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1613-6810, 1613-6829
KITopen-ID: 1000150270
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Small
Verlag John Wiley and Sons
Band 18
Heft 35
Seiten Art.-Nr.: 2202704
Vorab online veröffentlicht am 07.08.2022
Nachgewiesen in Dimensions
Scopus
Web of Science
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