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Pluripotency factors regulate the onset of Hox cluster activation in the early embryo

Tiana, María; Lopez-Jimenez, Elena; de Aja, Julio Sainz; Barral, Antonio; Victorino, Jesus; Badia-Careaga, Claudio; Rollan, Isabel; Rouco, Raquel; Santos, Elisa; Sanchez-Iranzo, Hector ORCID iD icon 1; Acemel, Rafael D.; Torroja, Carlos; Adan, Javier; Andres-Leon, Eduardo; Gomez-Skarmeta, Jose Luis; Giovinazzo, Giovanna; Sanchez-Cabo, Fatima; Manzanares, Miguel
1 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract:

Pluripotent cells are a transient population of the mammalian embryo dependent on transcription factors, such as OCT4 and NANOG, which maintain pluripotency while suppressing lineage specification. However, these factors are also expressed during early phases of differentiation, and their role in the transition from pluripotency to lineage specification is largely unknown. We found that pluripotency factors play a dual role in regulating key lineage specifiers, initially repressing their expression and later being required for their proper activation. We show that Oct4 is necessary for activation of HoxB genes during differentiation of embryonic stem cells and in the embryo. In addition, we show that the HoxB cluster is coordinately regulated by OCT4 binding sites located at the 3′ end of the cluster. Our results show that core pluripotency factors are not limited to maintaining the precommitted epiblast but are also necessary for the proper deployment of subsequent developmental programs.


Verlagsausgabe §
DOI: 10.5445/IR/1000150981
Veröffentlicht am 24.09.2022
Originalveröffentlichung
DOI: 10.1126/sciadv.abo3583
Scopus
Zitationen: 9
Web of Science
Zitationen: 8
Dimensions
Zitationen: 13
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 2375-2548
KITopen-ID: 1000150981
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Science Advances
Verlag American Association for the Advancement of Science (AAAS)
Band 8
Heft 28
Seiten 1-13
Vorab online veröffentlicht am 15.07.2022
Nachgewiesen in Scopus
Web of Science
Dimensions
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