KIT | KIT-Bibliothek | Impressum | Datenschutz

A matter of origin - identification of SEMA3A, BGLAP, SPP1 and PHEX as distinctive molecular features between bone site-specific human osteoblasts on transcription level

Zhang, Weiping; Rau, Sibylle; Kotzagiorgis, Konstantinos; Rothweiler, René; Nahles, Susanne; Gottwald, Eric ORCID iD icon 1; Rolauffs, Bernd; Steinberg, Thorsten; Nelson, Katja; Altmann, Brigitte
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)

Abstract:

In oral and maxillofacial bone reconstruction, autografts from the iliac crest represent the gold standard due to their superior clinical performance, compared to autografts derived from other extraoral regions. Thus, the aim of our study was to identify putative differences between osteoblasts derived from alveolar (hOB-A) and iliac crest (hOB-IC) bone of the same donor (nine donors) by means of their molecular properties in 2D and 3D culture. We thereby focused on the gene expression of biomarkers involved in osteogenic differentiation, matrix formation and osteoclast modulation. Furthermore, we examined the transcriptional response to Vit.D3 in hOB-A and hOB-IC. Our results revealed different modulation modes of the biomarker expression in osteoblasts, namely cell origin/bone entity-dependent, and culture configuration- and/or time-dependent modulations. SEMA3A, SPP1, BGLAP and PHEX demonstrated the strongest dependence on cell origin. With respect to Vit.D3-effects, BGLAP, SPP1 and ALPL displayed the highest Vit.D3-responsiveness. In this context we demonstrated that the transcriptional Vit.D3-response concerning SPP1 and ALPL in human osteoblasts depended on the cell origin. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000151074
Veröffentlicht am 28.09.2022
Originalveröffentlichung
DOI: 10.3389/fbioe.2022.918866
Scopus
Zitationen: 4
Web of Science
Zitationen: 4
Dimensions
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000151074
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 10
Seiten Art.Nr. 918866
Vorab online veröffentlicht am 28.09.2022
Schlagwörter osteoblasts, SEMA3, transcription
Nachgewiesen in Scopus
Dimensions
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page