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Highly Luminescent Positively Charged Quantum Dots Interacting with Proteins and Cells

Wang, Haixia 1; Nienhaus, Karin 1; Shang, Li 1; Nienhaus, Gerd Ulrich ORCID iD icon 1,2,3
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
3 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract:

We have studied interactions between positively charged MUTAB-stabilized quantum dots (QDs) and model proteins, serum and live cells using fluorescence correlation spectroscopy (FCS), dynamic light scattering (DLS), time-resolved photoluminescence (PL) and live-cell fluorescence imaging. Using human serum albumin (HSA) as a model protein, we measured the growth of a protein adsorption layer (“protein corona”) via time-resolved FCS. Corona formation was characterized by an apparent equilibrium dissociation coefficient, K$_{D}$ ≈ 10 μM. HSA adlayer growth was surprisingly slow (timescale ca. 30 min), in stark contrast to many similar measurements with HSA and other proteins and different NPs. Time-resolved PL data revealed a characteristic quenching behavior depending on the QD surface coverage with HSA. Taken together, we found that MUTAB-QDs initially bind HSA molecules weakly (K$_{D}$ ≈ 700 μM); however, the affinity is enhanced over time, presumably due to proton injection into the MUTAB layer by HSA triggering ligand dissociation. This process was also observed with human blood serum, showing equal kinetics for comparable HSA concentration. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000151270
Veröffentlicht am 21.10.2022
Originalveröffentlichung
DOI: 10.1002/cjoc.202200350
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
Dimensions
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Biologische und Chemische Systeme (IBCS)
Institut für Nanotechnologie (INT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1001-604X, 1614-7065
KITopen-ID: 1000151270
HGF-Programm 43.32.02 (POF IV, LK 01) Designed Optical Materials
Weitere HGF-Programme 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Chinese Journal of Chemistry
Verlag Chinese Chemical Society
Band 40
Heft 22
Seiten 2685-2693
Vorab online veröffentlicht am 13.09.2022
Nachgewiesen in Web of Science
Scopus
Dimensions
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