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Enhanced Bioactivity of Tailor-Made Glycolipid Enriched Manuka Honey

Delavault, André 1; Zoheir, Ahmed E. 2; Muller, Delphine 3; Hollenbach, Rebecca 1; Rabe, Kersten S. ORCID iD icon 2; Ochsenreither, Katrin ORCID iD icon 1; Rudat, Jens 1; Syldatk, Christoph 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)
3 Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Glycolipids can be synthetized in deep eutectic solvents (DESs) as they possess low water content allowing a reversed lipase activity and thus enables ester formation. Based on this principle, honey can also serve as a media for glycolipid synthesis. Indeed, this supersaturated sugar solution is comparable in terms of physicochemical properties to the sugar-based DESs. Honey-based products being commercially available for therapeutic applications, it appears interesting to enhance its bioactivity. In the current work, we investigate if enriching medical grade honey with in situ enzymatically-synthetized glycolipids can improve the antimicrobial property of the mixture. The tested mixtures are composed of Manuka honey that is enriched with octanoate, decanoate, laurate, and myristate sugar esters, respectively dubbed GOH, GDH, GLH, and GMH. To characterize the bioactivity of those mixtures, first a qualitative screening using an agar well diffusion assay has been performed with methicillin-resistant Staphylococcus aureus, Bacillus subtilis, Candida bombicola, Escherichia coli, and Pseudomonas putida which confirmed considerably enhanced susceptibility of these micro-organisms to the different glycolipid enriched honey mixtures. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000151352
Veröffentlicht am 12.10.2022
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1422-0067
KITopen-ID: 1000151352
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in International Journal of Molecular Sciences
Verlag MDPI
Band 23
Heft 19
Seiten Art.-Nr.: 12031
Bemerkung zur Veröffentlichung This article belongs to the Special Issue Enzymatic Synthesis of Novel and Bioactive Compounds.
Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 10.10.2022
Schlagwörter glycolipid synthesis; Manuka honey; lipase; esterification; deep eutectic solvent; antimicrobial; stress biosensing
Nachgewiesen in Web of Science
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