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Effect of process mode, nitrogen source and temperature on L-malic acid production with Aspergillus oryzae DSM 1863 using acetate as carbon source

Kövilein, Aline 1; Zadravec, Lena 2; Hohmann, Silja 2; Umpfenbach, Julia 2; Ochsenreither, Katrin ORCID iD icon 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)
2 Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Malic acid, mainly used as acidulant and taste enhancer in the food industry, is currently produced from fossil resources. In this study, microbial L-malate production with the filamentous fungus A. oryzae using the carbon source acetate was evaluated. Acetate is for example contained in biomass-derived substrates such as lignocellulosic hydrolysates and condensates of fast pyrolysis, thus avoiding competition with food production. Since research on malic acid synthesis from acetate is limited and reported productivities and yields are low, this work aimed to improve the process. First, different cultivation temperatures were tested. This parameter was found to affect the ratio between malic and succinic acid, which is the major by-product of organic acid production with A. oryzae. At 32°C, the malate share was highest (53.7 ± 1.6%), while it was lowest at 38°C (43.3 ± 1.1%) whereas succinate represented the main product (51.5 ± 1.0%). Besides the temperature, the type of nitrogen source was also found to affect malate synthesis as well as biomass production. In the pre-culture, the biomass concentration was increased by a factor of 3.4–3.9, and germination started earlier with the complex nitrogen sources yeast extract, casein hydrolysate and peptone compared to the defined nitrogen source (NH4)2SO4. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000151520
Veröffentlicht am 14.10.2022
Originalveröffentlichung
DOI: 10.3389/fbioe.2022.1033777
Scopus
Zitationen: 4
Web of Science
Zitationen: 4
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000151520
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 10
Seiten Art.-Nr.: 1033777
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 14.10.2022
Schlagwörter malate, succinate, organic acid, filamentous fungus, fed-batch, repeated-batch
Nachgewiesen in Web of Science
Scopus
Dimensions
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