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Diamond Surfaces with Clickable Antifouling Polymer Coating for Microarray‐Based Biosensing

Kumar, Ravi 1; Yang, Bingquan 1; Barton, Jan; Stejfova, Miroslava; Schäfer, Andreas; König, Meike ORCID iD icon 2; Knittel, Peter; Cigler, Petr ; Hirtz, Michael ORCID iD icon 1
1 Institut für Nanotechnologie (INT), Karlsruher Institut für Technologie (KIT)
2 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)

Abstract:

Diamond enables the construction of various (bio)sensors, including those with quantum-based detection. However, bare diamond interfaces are susceptible to unspecific adhesion of proteins and other macromolecules from biological media or complex samples. This impairs selectivity in biosensing, leads to low signal-to-noise ratio in fluorescence-based applications, and introduces the need for blocking steps in incubation protocols. Here, a stable, protein-repellent, and clickable reactive polymer coating is introduced, abolishing unspecific protein adhesion while concurrently enabling covalent immobilization of functional compounds as recognition elements. The polymer coating has two segments, an antifouling poly(N-(2-hydroxypropyl) methacrylamide) and an alkyne-terminated poly(propargyl methacrylamide) providing the click functionality. The antifouling properties and click-reactivity of the polymers are demonstrated by selective protein binding assays on micropatterns written by microchannel cantilever spotting (µCS). The assays demonstrated the successful functionalization of both diamond and glass surfaces and the excellent antifouling properties of the polymer coating. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000151809
Veröffentlicht am 03.11.2022
Originalveröffentlichung
DOI: 10.1002/admi.202201453
Scopus
Zitationen: 5
Web of Science
Zitationen: 6
Dimensions
Zitationen: 8
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 22.11.2022
Sprache Englisch
Identifikator ISSN: 2196-7350
KITopen-ID: 1000151809
HGF-Programm 43.31.02 (POF IV, LK 01) Devices and Applications
Weitere HGF-Programme 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Advanced Materials Interfaces
Verlag John Wiley and Sons
Band 9
Heft 33
Seiten Art.Nr. 2201453
Vorab online veröffentlicht am 10.10.2022
Schlagwörter Project-ID: 2020-023-028480 (DPN)
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Scopus
Web of Science
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