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Surface characterisation reveals substrate suitability for Cyanobacterial phototaxis

Julius, Lourdes Albina Nirupa ORCID iD icon 1; Matter, Lukas 1; Schuergers, Nils; Lützenkirchen, Johannes ORCID iD icon 2; Trouillet, Vanessa 3; Gil-Díaz, Teba ORCID iD icon 2; Mamleyev, Emil R. ORCID iD icon 1; Wilde, Annegret; Badilita, Vlad 1; Korvink, Jan G. 1
1 Institut für Mikrostrukturtechnik (IMT), Karlsruher Institut für Technologie (KIT)
2 Institut für Nukleare Entsorgung (INE), Karlsruher Institut für Technologie (KIT)
3 Institut für Angewandte Materialien – Energiespeichersysteme (IAM-ESS), Karlsruher Institut für Technologie (KIT)

Abstract:

Cyanobacteria respond to light stimulation, activating localized assembly of type IV pili for motility. The resulting phototactic response is highly dependent on the nature of the incoming light stimulus, and the final motility parameters depend on the surface properties. Conventionally, phototaxis studies are carried out on hydrogel surfaces, such as agarose, with surface properties, that vary in time due to experimental conditions. This study considers five substrates, widely utilized in microfluidic technology, to identify the most suitable alternative for performing reliable and repeatable phototaxis assays. The surfaces are characterized via a contact angle goniometer to determine the surface energy, white light interferometry for roughness, zeta-potentials and AFM force distance curves for charge patterns, and XPS for surface composition. Cell motility assays showed 1.25 times increment on surfaces with a water contact angle of 80 compared to a reference glass surface. To prove that motility can be enhanced, polydimethylsiloxane (PDMS) surfaces were plasma treated to alter their surface wettability. The motility on the plasma-treated PDMS showed similar performance as for glass surfaces. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000152401
Veröffentlicht am 13.12.2022
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Materialien – Energiespeichersysteme (IAM-ESS)
Institut für Mikrostrukturtechnik (IMT)
Institut für Nukleare Entsorgung (INE)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1742-7061
KITopen-ID: 1000152401
HGF-Programm 43.35.02 (POF IV, LK 01) Functionality of Soft Matter and Biomolecular Systems
Erschienen in Acta Biomaterialia
Verlag Elsevier
Band 155
Seiten 386-399
Vorab online veröffentlicht am 22.10.2022
Nachgewiesen in Dimensions
Web of Science
Scopus
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