Anatomical adjustments of the tree hydraulic pathway decrease canopy conductance under long-term elevated CO${}_2$

The cause of reduced leaf-level transpiration under elevated CO${}_2$ remains largely elusive. Here, we assessed stomatal, hydraulic, and morphological adjustments in a long-term experiment on Aleppo pine (Pinus halepensis) seedlings germinated and grown for 22–40 months under elevated (eCO${}_2$; c. 860 ppm) or ambient (aCO${}_2$; c. 410 ppm) CO${}_2$. We assessed if eCO${}_2$-triggered reductions in canopy conductance (g${}_c$) alter the response to soil or atmospheric drought and are reversible or lasting due to anatomical adjustments by exposing eCO${}_2$ seedlings to decreasing [CO${}_2$]. To quantify underlying mechanisms, we analyzed leaf abscisic acid (ABA) level, stomatal and leaf morphology, xylem structure, hydraulic efficiency, and hydraulic safety. Effects of eCO${}_2$ manifested in a strong reduction in leaf-level g${}_c$ (−55%) not caused by ABA and not reversible under low CO${}_2$ (c. 200 ppm). Stomatal development and size were unchanged, while stomatal density increased (+18%). An increased vein-to-epidermis distance (+65%) suggested a larger leaf resistance to water flow. This was supported by anatomical adjustments of branch xylem having smaller conduits (−8%) and lower conduit lumen fraction (−11%), which resulted in a lower specific conductivity (−19%) and leaf-specific conductivity (−34%). ... mehrThese adaptations to CO${}_2$ did not change stomatal sensitivity to soil or atmospheric drought, consistent with similar xylem safety thresholds. In summary, we found reductions of g${}_c$ under elevated CO${}_2$ to be reflected in anatomical adjustments and decreases in hydraulic conductivity. As these water savings were largely annulled by increases in leaf biomass, we do not expect alleviation of drought stress in a high CO${}_2$ atmosphere.