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Mixotrophic chain elongation with syngas and lactate as electron donors

Baleeiro, Flávio C. F. ORCID iD icon 1; Raab, Jana; Kleinsteuber, Sabine; Neumann, Anke 1; Sträuber, Heike
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Feeding microbial communities with both organic and inorganic substrates can improve sustainability and feasibility of chain elongation processes. Sustainably produced H2, CO2, and CO can be co-fed to microorganisms as a source for acetyl-CoA, while a small amount of an ATP-generating organic substrate helps overcome the kinetic hindrances associated with autotrophic carboxylate production. Here, we operated two semi-continuous bioreactor systems with continuous recirculation of H2, CO2, and CO while co-feeding an organic model feedstock (lactate and acetate) to understand how a mixotrophic community is shaped during carboxylate production. Contrary to the assumption that H2, CO2, and CO support chain elongation via ethanol production in open cultures, significant correlations (p < 0.01) indicated that relatives of Clostridium luticellarii and Eubacterium aggregans produced carboxylates (acetate to n-caproate) while consuming H2, CO2, CO, and lactate themselves. After 100 days, the enriched community was dominated by these two bacteria coexisting in cyclic dynamics shaped by the CO partial pressure. Homoacetogenesis was strongest when the acetate concentration was low (3.2 g L−1), while heterotrophs had the following roles: Pseudoramibacter, Oscillibacter, and Colidextribacter contributed to n-caproate production and Clostridium tyrobutyricum and Acidipropionibacterium spp. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000152939
Originalveröffentlichung
DOI: 10.1111/1751-7915.14163
Scopus
Zitationen: 4
Web of Science
Zitationen: 5
Dimensions
Zitationen: 5
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 1751-7915
KITopen-ID: 1000152939
HGF-Programm 38.05.01 (POF IV, LK 01) Anthropogenic Carbon Cycle
Erschienen in Microbial Biotechnology
Verlag Wiley Open Access
Band 16
Heft 2
Seiten 322-336
Vorab online veröffentlicht am 15.11.2022
Nachgewiesen in Web of Science
Dimensions
Scopus
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