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Band-selective universal 90° and 180° rotation pulses covering the aliphatic carbon chemical shift range for triple resonance experiments on 1.2 GHz spectrometers

Slad, Stella 1; Bermel, Wolfgang; Kümmerle, Rainer; Mathieu, Daniel; Luy, Burkhard ORCID iD icon 1
1 Institut für Organische Chemie (IOC), Karlsruher Institut für Technologie (KIT)

Abstract:

Biomolecular NMR spectroscopy requires large magnetic field strengths for high spectral resolution. Today’s highest fields comprise proton Larmor frequencies of 1.2 GHz and even larger field strengths are to be expected in the future. In protein triple resonance experiments, various carbon bandwidths need to be excited by selective pulses including the large aliphatic chemical shift range. When the spectrometer field strength is increased, the length of these pulses has to be decreased by the same factor, resulting in higher rf-amplitudes being necessary in order to cover the required frequency region. Currently available band-selective pulses like Q3/Q5 excite a narrow bandwidth compared to the necessary rf-amplitude. Because the maximum rf-power allowed in probeheads is limited, none of the selective universal rotation pulses reported so far is able to cover the full $^{13}$C aliphatic region on 1.2 GHz spectrometers. In this work, we present band-selective 90° and 180° universal rotation pulses (SURBOP90 and SURBOP180) that have a higher ratio of selective bandwidth to maximum rf-amplitude than standard pulses. Simulations show that these pulses perform better than standard pulses, e. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000153277
Veröffentlicht am 05.12.2022
Originalveröffentlichung
DOI: 10.1007/s10858-022-00404-1
Scopus
Zitationen: 6
Web of Science
Zitationen: 6
Dimensions
Zitationen: 7
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Organische Chemie (IOC)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2022
Sprache Englisch
Identifikator ISSN: 0925-2738, 1573-5001
KITopen-ID: 1000153277
HGF-Programm 43.35.02 (POF IV, LK 01) Functionality of Soft Matter and Biomolecular Systems
Erschienen in Journal of Biomolecular NMR
Verlag Springer
Band 76
Heft 5-6
Seiten 185–195
Vorab online veröffentlicht am 24.11.2022
Schlagwörter Triple resonance experiments, 1.2 GHz, Band-selective pulses, Universal rotations, Excitation, refocusing
Nachgewiesen in Scopus
Web of Science
Dimensions
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