Orthogonal protein decoration of DNA nanostructures based on SpyCatcher–SpyTag interaction
Kröll, Sandra
1; Schneider, Leonie 1; Wadhwani, Parvesh 1; Rabe, Kersten S.
1; Niemeyer, Christof M.
1
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)
1; Schneider, Leonie 1; Wadhwani, Parvesh 1; Rabe, Kersten S.
1; Niemeyer, Christof M.
11 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)
Abstract:
We present an efficient and readily applicable strategy for the covalent ligation of proteins to DNA origami by using the SpyCatcher–SpyTag (SC–ST) connector system. This approach showed orthogonality with other covalent connectors and has been used exemplarily for the immobilization and study of stereoselective ketoreductases to gain insight into the spatial arrangement of enzymes on DNA nanostructures.
| Zugehörige Institution(en) am KIT | Institut für Biologische Grenzflächen (IBG) |
| Publikationstyp | Zeitschriftenaufsatz |
| Publikationsdatum | 06.12.2022 |
| Sprache | Englisch |
| Identifikator | ISSN: 1359-7345, 0009-241X, 0022-4936, 1364-548X, 2050-5620, 2050-5639 KITopen-ID: 1000153608 |
| HGF-Programm | 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems |
| Weitere HGF-Programme | 47.14.01 (POF IV, LK 01) Molekular Information Processing in Cellular Systems |
| Erschienen in | Chemical Communications |
| Verlag | Royal Society of Chemistry (RSC) |
| Band | 58 |
| Heft | 97 |
| Seiten | 13471–13474 |
| Nachgewiesen in | Web of Science Dimensions OpenAlex Scopus |