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Identification of Zirconia Particle Uptake in Human Osteoblasts by ToF-SIMS Analysis and Particle-Size Effects on Cell Metabolism

Welle, Alexander ORCID iD icon 1,2; Rabel, Kerstin; Schwotzer, Matthias ORCID iD icon 1; Kohal, Ralf Joachim; Steinberg, Thorsten; Altmann, Brigitte
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
2 Karlsruhe Nano Micro Facility (KNMF), Karlsruher Institut für Technologie (KIT)


As the use of zirconia-based nano-ceramics is rising in dentistry, the examination of possible biological effects caused by released nanoparticles on oral target tissues, such as bone, is gaining importance. The aim of this investigation was to identify a possible internalization of differently sized zirconia nanoparticles (ZrNP) into human osteoblasts applying Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), and to examine whether ZrNP exposure affected the metabolic activity of the cells. Since ToF-SIMS has a low probing depth (about 5 nm), visualizing the ZrNP required the controlled erosion of the sample by oxygen bombardment. This procedure removed organic matter, uncovering the internalized ZrNP and leaving the hard particles practically unaffected. It was demonstrated that osteoblasts internalized ZrNP within 24 h in a size-dependent manner. Regarding the cellular metabolic activity, metabolization of alamarBlue by osteoblasts revealed a size- and time-dependent unfavorable effect of ZrNP, with the smallest ZrNP exerting the most pronounced effect. These findings point to different uptake efficiencies of the differently sized ZrNP by human osteoblasts. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000153707
Veröffentlicht am 14.12.2022
DOI: 10.3390/nano12234272
Zitationen: 2
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 01.12.2022
Sprache Englisch
Identifikator ISSN: 2079-4991
KITopen-ID: 1000153707
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Nanomaterials
Verlag MDPI
Band 12
Heft 23
Seiten Art.-Nr.: 4272
Bemerkung zur Veröffentlichung This article belongs to the Special Issue Recent Advances in the Assessment of Engineered Nanomaterials: Ecotoxicity, Cytotoxicity and Genotoxicity
Schlagwörter 2021-025-030109 ToF-SIMS, zirconia, nano/microparticles, Time-of-Flight Secondary Ion Mass Spectrometry, human alveolar bone, alamarBlue assay
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Web of Science
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